Journal: Cell reports

Article Title: DUX4 expression in cancer induces a metastable early embryonic totipotent program

doi: 10.1016/j.celrep.2023.113114

Figure Lengend Snippet:

Article Snippet: Fluorescein (FITC) AffiniPure Donkey Anti-Rat IgG , Jackson ImmunoResearch , Cat#712-095-150, RRID:AB_2340651.

Techniques: Recombinant, Electron Microscopy, Transfection, Bicinchoninic Acid Protein Assay, Amplification, SYBR Green Assay, Plasmid Preparation, DNA Purification, Control, Sequencing, Clone Assay, Software, Microscopy

Journal: Cell reports

Article Title: Axon targeting of Drosophila medulla projection neurons requires diffusible Netrin and is coordinated with neuroblast temporal patterning

doi: 10.1016/j.celrep.2023.112144

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Donkey Anti-Rat Cy5 , Jackson ImmunoResearch Laboratories Inc. , Cat# 712-175-153; RRID:AB_2340672.

Techniques: Recombinant, Software

Journal: Cell reports

Article Title: Endothelial Cells in the Decidual Bed Are Potential Therapeutic Targets for Preterm Birth Prevention

doi: 10.1016/j.celrep.2019.04.049

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cy3 AffiniPure Donkey Anti-Rat IgG (H+L) , Jackson ImmunoResearch , Cat#712-165-150; RRID:AB_2340666.

Techniques: Labeling, Recombinant, Software

Journal: iScience

Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers

doi: 10.1016/j.isci.2023.106310

Figure Lengend Snippet:

Article Snippet: The following secondary antibodies were used and incubated at room temperature for an hour: Peroxidase-conjugated goat anti-Armenian Hamster IgG (H + L) from Fitzgerald, Biotin-SP-AffiniPure Donkey Anti-Rat IgG (H + L) and Peroxidase-AffiniPure Donkey Anti-Rat IgG (H + L) from Jackson ImmunoResearch (Cambridge, UK).

Techniques: Purification, Recombinant, Virus, Saline, Plasmid Preparation, Microscopy, Reverse Transcription, Software

Journal: eLife

Article Title: CD31 signaling promotes the detachment at the uropod of extravasating neutrophils allowing their migration to sites of inflammation

doi: 10.7554/eLife.84752

Figure Lengend Snippet: ( A ) Absolute number of Ly6G + cells harvested in the peritoneal cavity at different time points after the induction of sterile peritonitis in WT or Pecam1 -/- mice. ( B ) Blood neutrophil levels at the indicated time points. ( C ) Concentration of SDF-1, CD62P, CXCL1, and PTX-3 in the plasma and cell-free peritoneal fluid of Pecam1 -/- and WT mice at 4 hr after PBS or IL-1β injection. Data are presented as mean ± SE. n = 3/5 per condition. The data represent three independent experiments. Statistical significance is indicated relative to the previous time point. Mann–Whitney test: *p<0.05, **p<0.01, ***p<0.001. ( D ) Confocal micrographs of whole-mount omentum showing extravasating Ly6G + cells (clone 1A8; green) from a post-capillary venule with CD31 expression (polyclonal, R&D #AF3628; white). Samples were also stained with a monoclonal antibody against phosphorylated CD31 tyrosine 713 (pY713, clone EPR8079; red staining). Inset shows colocalization of pY713 and Ly6G. Scale bar 50 µm. ( E ) Inverse correlation between pY713 staining intensity of each Ly6G + cell and the distance from the vessel (Spearman correlation and exponential one-phase decay regression with 95% confidence interval). ( F ) Quantification of neutrophil distance from the closest vessel in WT and Pecam1 -/- mice 4 hr after IL-1β injection. n = 153 and n = 89 cells for WT and Pecam1 -/- mice, respectively. Unpaired nonparametric Mann–Whitney test: ***p<0.001. ( G ) Ly6G + cells (green) accumulate around the outer edge of post-capillary venules (phalloidin staining, white) in the omentum of Pecam1 -/- mice. Scale bar 50 µm. ( H ) Percentage of neutrophils attached to the outer part of the vessels (gray) and percentage of neutrophils released into the extravascular space (black) in WT and Pecam1 -/- mice. Figure 1—source data 1. Quantification of total number of neutrophils in WT and Pecam1 -/- mice after IL-1β challenging . Figure 1—source data 2. Fraction of neutrophils in the peripheral blood of WT and Pecam1 -/- mice after IL-1β challenging . Figure 1—source data 3. Quantification of inflammatory cytokines in the plasma or peritoneal fluid of WT and Pecam1 -/- mice 4 hr after IL-1β challenging . Figure 1—source data 4. Spearman correlation between the distance of each neutrophil from the closest capillary venule and its CD31 phospho(p)-Tyrosine(Y)–713 level . Figure 1—source data 5. Distance (µm) of neutrophils from the closest capillary venule in the omentum of WT and Pecam1 -/- mice upon IL-1β challenge (n = 3/group) . Figure 1—source data 6. Fraction of neutrophils attached (<5 µm) or released (>5 µm) from the closest capillary venule in the omentum of WT and Pecam1 -/- mice upon IL-1β challenge (n = 3/group) .

Article Snippet: Antibody , Alexa Fluor 647 AffiniPure anti-rat IgG, (H+L), donkey polyclonal , Jackson ImmunoResearch , Cat# 712-605-153, RRID :AB_2340694 , 1:500.

Techniques: Sterility, Concentration Assay, Clinical Proteomics, Injection, MANN-WHITNEY, Expressing, Staining

Journal: eLife

Article Title: CD31 signaling promotes the detachment at the uropod of extravasating neutrophils allowing their migration to sites of inflammation

doi: 10.7554/eLife.84752

Figure Lengend Snippet: ( A–G ) Confocal microscopy phenotypic characterization of purified WT or Pecam1 -/- neutrophils placed on immobilized recombinant mouse laminin α4 and recombinant mouse CXCL1. ( A ) Representative confocal images and ( B, C ) quantification of talin (polyclonal, ab71333; red) and F-actin (Phalloidin, green) staining in WT mice ( B ) and Pecam1 -/- mice ( C ). ( D ) Quantification of the uropod lengths of WT and Pecam1 -/- neutrophils calculated as the distance from the backside of the nucleus to the end of the cellular tail. ( E ) Neutrophil total area. Data comes from three independent experiments, *p<0.05, **p<0.01, ***p<0.001 (unpaired Student’s t -test). ( F, G ) Quantification of CD44 (clone IM7; red) and SHIP1 (clone PC1C; green) staining in WT mice ( F ) and Pecam1 -/- mice ( G ). ( H, I ) Quantification of talin (red) and F-actin (Phalloidin, green) staining in Pecam1 ITIM+/+ mice ( H ) and Pecam1 ITIM-/- ( I ). Quantification of talin/phalloidin ratio at the front ( J ) and back ( K ) edge of analyzed cells. Quantification of mean fluorescence intensities (MFIs) of the indicated staining along the cell axis (mean ±SE [SE bars in gray color]; n > 8 cells/staining; statistical comparisons between the 20 first frontal and 20 last posterior measurement points are shown). *p<0.05, **p<0.01, ***p<0.001 (Mann–Whitney test). Figure 3—source data 1. Quantification of mean fluorescence intensities (MFIs) of polymerized F-actin (phalloidin) and Talin (polyclonal ab71333) along the cell axis of WT neutrophils . Figure 3—source data 2. Quantification of mean fluorescence intensities (MFIs) of polymerized F-actin (phalloidin) and Talin (polyclonal ab71333) along the cell axis of Pecam1 -/- neutrophils . Figure 3—source data 3. Uropod length (µm) of WT and Pecam1 -/- neutrophils migrating on laminin α4-coated surfaces . Figure 3—source data 4. Cell area (µm 2 ) of WT and Pecam1 -/- neutrophils migrating on laminin α4-coated surfaces . Figure 3—source data 5. Quantification of mean fluorescence intensities (MFIs) of CD44 (clone IM7) and SHIP-1 (clone PC1C) along the cell axis of WT neutrophils . Figure 3—source data 6. Quantification of mean fluorescence intensities (MFIs) of CD44 (clone IM7) and SHIP-1 (clone PC1C) along the cell axis of Pecam1 -/- neutrophils . Figure 3—source data 7. Quantification of mean fluorescence intensities (MFIs) of polymerized F-actin (phalloidin) and Talin (polyclonal ab71333) along the cell axis of WT Pecam1 ITIM-/- neutrophils . Figure 3—source data 8. Quantification of mean fluorescence intensities (MFIs) of polymerized F-actin (phalloidin) and Talin (polyclonal ab71333) along the cell axis of Pecam1 ITIM-/- neutrophils . Figure 3—source data 9. Quantification of polymerized F-actin (phalloidin) and Talin (polyclonal ab71333) ratio at the leading edge of WT and Pecam1 ITIM-/- neutrophils . Figure 3—source data 10. Quantification of polymerized F-actin (phalloidin) and Talin (polyclonal ab71333) ratio at the uropod of WT and Pecam1 ITIM-/- neutrophils .

Article Snippet: Antibody , Alexa Fluor 647 AffiniPure anti-rat IgG, (H+L), donkey polyclonal , Jackson ImmunoResearch , Cat# 712-605-153, RRID :AB_2340694 , 1:500.

Techniques: Confocal Microscopy, Purification, Recombinant, Staining, Fluorescence, MANN-WHITNEY

Journal: eLife

Article Title: CD31 signaling promotes the detachment at the uropod of extravasating neutrophils allowing their migration to sites of inflammation

doi: 10.7554/eLife.84752

Figure Lengend Snippet:

Article Snippet: Antibody , Alexa Fluor 647 AffiniPure anti-rat IgG, (H+L), donkey polyclonal , Jackson ImmunoResearch , Cat# 712-605-153, RRID :AB_2340694 , 1:500.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Western Blot, Generated, Software, Flow Cytometry, Mass Spectrometry, Microscopy

Journal: iScience

Article Title: Salmonella infection induces the reorganization of follicular dendritic cell networks concomitant with the failure to generate germinal centers

doi: 10.1016/j.isci.2023.106310

Figure Lengend Snippet:

Article Snippet: The following secondary antibodies were used and incubated at room temperature for an hour: Peroxidase-conjugated goat anti-Armenian Hamster IgG (H + L) from Fitzgerald, Biotin-SP-AffiniPure Donkey Anti-Rat IgG (H + L) and Peroxidase-AffiniPure Donkey Anti-Rat IgG (H + L) from Jackson ImmunoResearch (Cambridge, UK).

Techniques: Purification, Recombinant, Virus, Saline, Plasmid Preparation, Microscopy, Reverse Transcription, Software